A simple and reliable swab-based test for the detection of Salmonella spp. on food contact surfaces and in processing environments. Ideal for ensuring regulatory compliance and minimising the risk of contamination.
Highly sensitive, easy-to-use, and impressively quick, the test uses XLT-4 medium to highlight the presence of Salmonella spp. through a distinct colour change.
Intended Use
The Salmonella Isolation Swab uses a modified XLT41 (Xylose-lysine-tergitol 4) medium to differentiate between Salmonella spp. and non-Salmonella spp. XLT-4 Medium was developed for the selective identification of Salmonella enteritidis from environmental specimens containing large numbers of competing organisms.
This swab-based test is ideal for sampling food contact surfaces and processing environments, offering accurate and reliable results.
Why choose Klipspringer Environmental Swabbing Tests?
- Highly sensitive, so will detect even low levels of the target organism
- Organisms remain viable for further testing
- Easy to use, with highly visible results
- Fast results, helping to speed up the process of taking corrective action
Instructions for Use
- Peel back wrapper to expose both caps.
- Remove swab and sample test site.
- Remove cap of culture tube with thumb and forefinger and discard.
- Insert swab in culture tube and push down fully to immerse the swab completely.
- Fill in site details, etc.
- Incubate at 37°C for 24-48 hours. If no colour change is observed after 24 hours, reincubate for a further 24 hours to allow lower levels of detected organisms to grow.
- Read results.
- Dispose of tube.
Precautions
- If any orange or black/yellow media tubes are found when the box is opened, DO NOT USE.
Precautions When Swabbing
- When sampling an area always use the same swabbing technique.
- Always try to sample the same surface are (100 cm² or 200 cm²), and be consistent in your sampling.
- The moisture level on the surface should not vary at different sample times. It may be preferable with dry surfaces to wet the swab in a suitable wetting agent such as Ringers, Tween Lecithin Thiosulphate Ringers or Tween Peptone water.
Example Of Area To Be Swabbed

Visual Result
Expected Results Based on a 104 Colony Forming Unit Inoculation
Organism | 24hrs Colour Change | 48hrs Colour Change |
---|---|---|
E. Coli | No colour change1 | No colour change1 |
Proteus Mirabilis | No colour change1 | No colour change1 |
Enterococcus faecalis | No colour change1 | No colour change1 |
Salmonella enteritidis | Slight change around swab some black pigmentation2 | Black colour change red media with black pigmentation all around swab3 |
Salmonella typhimurium | Slight change around swab some black pigmentation2 | Colour change red media with some black pigmentation around swab3 |
Enterobacter zakazakii | Slight colour change media turning orange around swab4 | Colour change all media has turned orange5 |
Staphylococcus aureus | No colour change1 | No colour change1 |
Citrobacter freundii | Colour change orange media with black pigmentation all around swab6 | Colour change orange media with black pigmentation all around swab6 |
Pseudomonas aeruginosa | No colour change1 | No colour change1 |
Listeria monocytogenes | No colour change1 | No colour change1 |
- 12 months from the date of manufacture.
- The expiration date is printed on the tube label, peel pouch, and box label.
Notes 1-6 refer to the below colour chart
Note | Appearance of swab |
---|---|
1 | ![]() |
2 | ![]() |
3 | ![]() |
4 | ![]() |
5 | ![]() |
6 | ![]() |
Storage
Store the tests at 5-25°C. Do not freeze.