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Salmonella Tests

A simple and reliable swab-based test for the detection of Salmonella spp. on food contact surfaces and in processing environments. Ideal for ensuring regulatory compliance and minimising the risk of contamination.

Highly sensitive, easy-to-use, and impressively quick, the test uses XLT-4 medium to highlight the presence of Salmonella spp. through a distinct colour change. 

Code: WPG7439
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Code: WPG7439
Availability 4

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Intended Use

The Salmonella Isolation Swab uses a modified XLT41 (Xylose-lysine-tergitol 4) medium to differentiate between Salmonella spp. and non-Salmonella spp. XLT-4 Medium was developed for the selective identification of Salmonella enteritidis from environmental specimens containing large numbers of competing organisms. 

This swab-based test is ideal for sampling food contact surfaces and processing environments, offering accurate and reliable results.

Why choose Klipspringer Environmental Swabbing Tests?

  • Highly sensitive, so will detect even low levels of the target organism
  • Organisms remain viable for further testing
  • Easy to use, with highly visible results
  • Fast results, helping to speed up the process of taking corrective action

Instructions for Use

  1. Peel back wrapper to expose both caps.
  2. Remove swab and sample test site.
  3. Remove cap of culture tube with thumb and forefinger and discard.
  4. Insert swab in culture tube and push down fully to immerse the swab completely.
  5. Fill in site details, etc.
  6. Incubate at 37°C for 24-48 hours. If no colour change is observed after 24 hours, reincubate for a further 24 hours to allow lower levels of detected organisms to grow.
  7. Read results.
  8. Dispose of tube.

Precautions

  • If any orange or black/yellow media tubes are found when the box is opened, DO NOT USE.

Precautions When Swabbing

  • When sampling an area always use the same swabbing technique.
  • Always try to sample the same surface are (100 cm² or 200 cm²), and be consistent in your sampling.
  • The moisture level on the surface should not vary at different sample times. It may be preferable with dry surfaces to wet the swab in a suitable wetting agent such as Ringers, Tween Lecithin Thiosulphate Ringers or Tween Peptone water.

Example Of Area To Be Swabbed

Visual Result

Expected Results Based on a 104 Colony Forming Unit Inoculation

Organism 24hrs Colour Change 48hrs Colour Change
E. Coli No colour change1 No colour change1
Proteus Mirabilis No colour change1 No colour change1
Enterococcus faecalis No colour change1 No colour change1
Salmonella enteritidis Slight change around swab some black pigmentation2 Black colour change red media with black pigmentation all around swab3
Salmonella typhimurium Slight change around swab some black pigmentation2 Colour change red media with some black pigmentation around swab3
Enterobacter zakazakii Slight colour change media turning orange around swab4 Colour change all media has turned orange5
Staphylococcus aureus No colour change1 No colour change1
Citrobacter freundii Colour change orange media with black pigmentation all around swab6 Colour change orange media with black pigmentation all around swab6
Pseudomonas aeruginosa No colour change1 No colour change1
Listeria monocytogenes No colour change1 No colour change1

      Notes 1-6 refer to the below colour chart

    Note Appearance of swab
    1
    2 Salmonella Swab 2
    3 Salmonella Swab 3
    4 Salmonella Swab 4
    5 Salmonella Swab 5
    6 Salmonella Swab 6


    Storage

    Store the tests at 5-25°C. Do not freeze.

    Shelf Life

    • 12 months from the date of manufacture.
    • The expiration date is printed on the tube label, peel pouch, and box label.